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| Registros recuperados: 11 | |
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| Vargas Júnior,Sergio Farias; Costa,Vitória Gasperin Guazzelli; Oliveira,Fernando Caetano de; Goularte,Karina Lemos; Haas,Cristina Sangoi; Gasperin,Bernardo Garziera; Lucia Júnior,Thomaz. |
| Abstract: The objective of this work was to evaluate sperm cell motility after intrafollicular artificial insemination (IFAI) in vivo or after incubation in follicular fluid in vitro. In the in vivo experiment, IFAI was performed, followed by the recovery of follicular content 1 to 4 hours later, in order to assess sperm motility. In the in vitro experiment, spermatozoa from a pool of commercial frozen-thawed semen were evaluated for their kinetics after incubation for 1 or 3 hours, either pure (pool, control group) or in follicular fluid (FF). A low motility of sperm cells was observed in the FF samples, both in vitro and in vivo. In vitro, the main parameters negatively affected in the sperm cells incubated in FF, compared with the control, were: total... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Infertility; Insemination; Intrafollicular artificial insemination; Spermatozoa. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2018000700866 |
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| Fauvel, Christian; Suquet, Marc; Dreanno, Catherine; Zonno, Vincenzo; Menu, Bruno. |
| A sperm cryopreservation protocol adapted from turbot, was tested on sea bass using either 250-mu L straws or 1.5-mL cryovials. A dilution to 1/3 in Mounib's extender and a cooling rate of 65 degrees C.min(-1) allowed frozen sperm to recover an initial motility similar to that of fresh sperm at thawing; however, significant differences in motility (P < 0.001, n = 10 fish semen) were observed at further post-activation times, the motility decrease being faster in thawed sperm. At the experimental scale, triplicate inseminations of 2-mL aliquots (approximately 2 000 eggs) showed a significant fertility decay of thawed sperm compared to that of fresh sperm (P < 0.01, n = 12 fish semen) when a discriminating 35.10(3) spermatozoa to egg ratio was applied.... |
| Tipo: Text |
Palavras-chave: Dicentrarchus labrax; Sea bass; Cryobank; Insemination; Spermatozoa; Cryopreservation; Dicentrarchus labrax; Loup; Banque de sperme; Insémination; Cryoconservation; Cryoconservation. |
| Ano: 1998 |
URL: http://archimer.ifremer.fr/doc/1998/publication-866.pdf |
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| Suquet, Marc; Dreanno, Catherine; Fauvel, Christian; Cosson, J; Billard, R. |
| Since the first work of Blaxter in 1953, fish sperm cryopreservation has been attempted on about 30 marine species. The present paper reviews the techniques used and the results published in these species. Particular attention is paid to the handling procedure of sperm before freezing, the problems of semen ageing and semen contamination with urine. The quality of frozen-thawed semen was evaluated using previously standardized biotests, such as a two-step motility activation technique adapted for the different species and fertilization assays using a discriminating insemination technique. Most extenders used in marine fish are saline or sugar solutions. From the investigated cryoprotectants, dimethyl sulphoxide (DMSO) generally leads to the best results.... |
| Tipo: Text |
Palavras-chave: DMSO; Insemination; Fertilization; Cryopreservation; Fish sperm. |
| Ano: 2000 |
URL: http://archimer.ifremer.fr/doc/2000/publication-611.pdf |
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| Grande,Paula Adriana; Santos,Geraldo Tadeu dos; Ribeiro,Hilton; Damasceno,Júlio César; Alcalde,Claudete Regina; Barbosa,Orlando Rus; Horst,José Augusto; Santos,Fabio Seiji dos. |
| The effects of farm, parity (PO) and month of parturition on milk production, percentage of fat and protein, somatic cell count (SCC), and milk urea nitrogen (MUN) concentration were assessed on four specialized dairy farms using 120 cows. Also, the effects of MUN on gestation rate (GR) and artificial insemination rate (AIR) in early lactation were studied. The parameters of production and milk quality were similar among farms and were not influenced by the month of parturition. Farm D presented the highest MUN concentration. Concentration of MUN, percentages of fat and protein and SCC were not influenced by PO. Cows with a value of MUN between 10.1 and 13.0 mg/dL had the highest AIR and GR between days 55 and 70 postpartum and after 70 days in milk. These... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Dairy cattle; Gestation; Insemination; Milk urea nitrogen; Parturition order. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000700032 |
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| Loiola,Marcus Vinícius Galvão; Bittencourt,Rodrigo Freitas; Rodrigues,Alexandra Soares; Ferraz,Priscila Assis; Lima,Marcos Chalhoub Coelho; Carvalho,Caio Victor Damasceno; Ribeiro Filho,Antonio de Lisboa. |
| Abstract: The objective of this work was to evaluate the effects of supplementation with melengestrol acetate (MGA), after timed artificial insemination (TAI), on the conception rate of Nellore cows, according to cyclicity during the protocol, estrus expression between intravaginal device withdrawal and insemination, and diameter of the largest follicle at the time of insemination, in order to identify predictors of the use of MGA. Multiparous lactating cows (577) were subjected to a hormonal protocol for ovulation synchronization. On day -2 of the protocol, the animals were painted with a marker on the base of their tail. On day 0, they were evaluated for estrus expression; the preovulatory follicle diameter (POFD) was measured by ultrasonography, and the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Anestrous; Bovine; Fertility; Insemination; Melengestrol acetate. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2018000100105 |
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| Fukakusa,Clayton Kunio; Mazzoni,Talita Sarah; Malabarba,Luiz Roberto. |
| Abstract Most teleosts are externally fertilizing, with internal fertilization occurring as a relatively rare event. Until now, Euteleosteomorpha is the only teleost cohort known to undergo internal fertilization. In the teleost cohort Otomorpha, it has been recorded the presence of sperm in the ovaries of some species of Characiformes and Siluriformes, but no fertilized eggs have been found so far in the female reproductive tract. It has been presumed that oocytes can be released into the water with associated spermatozoa and only there becomes fertilized, and the term insemination has been used to characterize the strategy adopted by these fish. Here, we present the discovery of the first case of internal fertilization in the teleost cohort Otomorpha, in... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Characiformes; Insemination; Ostariophysi; Reproduction; Spawning. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252020000100209 |
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| Registros recuperados: 11 | |
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